20Med PNP platform
Ready-to-use delivery platform for intracellular delivery of nucleotide-based payloads.
20Med PNP composition
20Med nanoparticles are composed of bio-responsive poly(amido)amines (PAA) specifically modified for efficient intracellular delivery of nucleotide-based payloads such as oligonucleotides and mRNA or DNA. The polymers provide enhanced stability and increased biological effectivity to the system.
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20Med nanoparticles are formed when the polymeric components are combined with the nucleotide payload in a single and highly efficient formulation step. Solvents are not used for the formulation of the polymers with the payload which leads to a maximum yield since additional purification steps are not needed.
20Med PNP technology
20Med's proprietary bio-responsive polymeric nanoparticles provide protection of fragile payloads after administration but allow for rapid bio-responsive release of that payload as soon as the particles enter the cytosol.
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Cellular uptake - 20Med nanoparticles interact with the cellular membrane and are taken up into the cells via endocytosis.
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Endosomal escape - in order to deliver the cargo successfully to the nucleus, the 20Med Nanoparticles escape from the endosome by a process called the "proton sponge effect".
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Bioresponsive degradation - after endosomal escape, the 20Med nanoparticles are located in the cytoplasm. The glutathione in the cytosol breaks down the disulfide bonds in the polymers and enable release of the payload into the cytosol.
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Release of payload into cytosol
